The government's preferred routes for the HS2 high speed railway north of Birmingham have been confirmed.
The eastern route to Leeds will run east of Sheffield, with some trains going into the city on existing lines.
As a result, 16 homes on the Shimmer housing estate in Mexborough are expected to be demolished, the Department for Transport (DfT) said.
The routes, from Crewe to Manchester and from the West Midlands to Leeds, were laid out last November.
Earlier the government announced seven contracts worth nearly £7bn for some of the line's civil engineering between Birmingham and London.
The department for Transport said the full line would connect Birmingham, Manchester, Liverpool, Leeds, Sheffield and the East Midlands, with all of them being linked to London by the line.
The Transport Secretary, Chris Grayling, said: "Britain's new railway line will bring huge economic benefits across the country and help ensure this government delivers on its promise to spread wealth beyond London and the south-east.